2.7. In Vitro Platelet Aggregation Assay

The platelet aggregation assay was performed with reference to the previous paper [20]. Platelet aggregation was induced using collagen (final concentration, 2 μg/mL) or the ADP receptor analogue peptide (final concentration, 5 µM) as agonists. PRP was placed in plastic cuvettes with stir bars, reacted at 37 °C for 10 min, and MJGE09 (0, 4, 20, and 100 μg/mL) or aspirin (0.1 mM) was added and reacted for 5 min. After confirming the baseline for approximately 1 min, a platelet aggregation-promoting substance (collagen or ADP) was added, and the result was measured at 37 °C for up to 10 min, while stirring at 1200 rpm. Platelet aggregation was measured using an aggregometer (Chrono-Log 700-2; Havertown, PA, USA).