Indomethacin/HCl-induced gastric ulcer model and dosing

Induction of the INDO/HCl-induced gastric ulcer model was performed according to a previously reported method, ⁷ with some modifications. After adaptation for 1 week, 6-week-old male ICR mice were divided randomly into the following six groups of equal numbers (n = 7), avoiding intergroup differences in body weight: the normal control group (CTL), the ulcer model control group, positive control group (PCTL), and the SR5 100, 150, and 200 mg/kg treated groups. The mice were restricted from food for 24 h and from water for 12 h prior to induction of gastric ulcers. The normal control group and ulcer model control groups were given distilled water, and the positive control group was given 36 mg/kg DA-5204 (Stillen 2X) by oral administration. The SR-5 groups were pretreated for 3 days with 100, 150, and 200 mg/kg SR-5 extract by oral administration. Every 2 h beginning 1 h after the pretreatment, 30 mg/mL indomethacin (200 μL/mouse) was orally administered four times for ulcer induction, as shown in Figure 1. Two hours after the final indomethacin administration, all groups were given 200 μL of 200 mM HCl. Animals were sacrificed by anesthetization with isoflurane 30 min after treatment with HCl. The entire stomach was removed immediately and examined for gross mucosal injury. The stomach was subsequently stored at −80°C.