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HHDPC was seeded onto 96-well microplate (3×104 cells/well) and incubated with different RGE concentrations (100, 200, 300, and 400 µg/mL) or water (control) for 24 h. MTT solution (5 mg/mL) was added to each well and cells were incubated at 37°C for 4 h in the dark. Purple formazan product, a metabolite of MTT, was then solubilized with 100 mL of dimethyl sulfoxide. The absorbance at 570 nm was measured using a microplate reader (BioTek Instruments, Winooski, VT, USA).
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