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Urine and blood parameters

MN Masumi Namba
TK Tomoe Kobayashi
MK Mayumi Kohno
TK Takayuki Koyano
TH Takuo Hirose
MF Masaki Fukushima
MM Makoto Matsuyama
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Urine samples were collected for 16 h by placed rats in metabolic cages individually, every 4 weeks over 52 weeks (see Supplementary Table 1). Proteinuria level was measured by a modified method using 3% sulfosalicylic acid. Blood samples (n = 6 each) were collected from the tail and centrifuged at 3000 g for 5 min to obtain blood serum. Blood urea nitrogen (BUN) of serum was measured using Colorimetric Detection Kit (Arbor Assays, Michigan, USA). Creatinine (Cre) levels of serum or urine were measured Jaffe’s method (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan). Creatinine clearance ratio was calculated by (urine creatinine [mg/dl] x urine volume [dl])/serum creatinine [mg/dl]. All measurements were carried out according to manufacturer’s recommended protocols.

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