Ba/F3 Allograft Experiment

Ba/F3 cells expressing HER2 exon 20^YVMA insertion mutation were cultured in RPMI1640 medium supplemented with 10% FBS and incubated at 37 °C with 5% CO₂. On the day of implantation, cells were harvested, re-suspended in serum-free RPMI 1640 and a 100 μL cell suspension (10⁷ cells) was implanted subcutaneously in the right flank of female severe combined immunodeficiency (SCID) mice. All mice were weighed prior to dosing and throughout the study once daily. The tumors were measured in 2 dimensions (length and width) at least twice per week with a caliper in millimeters. Tumor volume (mm³) was calculated with the following formula: tumor volume = L x W² x 0.5.