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MBo (Methyl diaminobenzene-BODIPY) was used to detect endogenous MG in different conditions. The cells were treated with 5 µM MBo in complete medium as previously described (Wang et al., 2013). After 1 hr, the cells were washed with PBS and incubated in low- or high-glucose medium for 6 (FACS) and 24 hr (confocal microscopy). Cells were either trypsinized and analyzed by flow cytometry (BD Biosciences FACSCanto), or fixed with PAF and prepared for confocal microscope visualization as described above.
Copyright and License information: ©2016, Nokin et al
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