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Cluster of differentiation (CD) 3 immunostaining was performed on several slides across the depth of each analyzed tumor block, for a total of 418 regions. Slides were digitally acquired at 20× resolution and loaded into QuPath7 to quantify the number of CD3+ cells/mm2. H&E staining was performed on 13 additional slides of the validation cohort.
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This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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