Extraction.

DNA was extracted using the above-described 1.5 McFarland bacterial isolate suspensions. Briefly, 200 μl of 1.5 McFarland suspension in TSB was added to 2 ml of NucliSENS easyMAG lysis buffer (bioMérieux) and incubated for 10 min, and then DNA was automatically extracted using the bioMérieux NucliSENS easyMAG nucleic acid extractor (bioMérieux), specific A protocol. DNA was eluted in 110 μl NucliSENS easyMAG elution buffer (bioMérieux), quantified using the Qubit 3.0 fluorometer (Life Technologies, Invitrogen) according to the manufacturer’s protocol for high-sensitivity double-stranded DNA (dsDNA), and stored at −20°C until use.