RNase R and Actinomycin D (ActD) treatment

FC Fei Cao
XW Xiaoxia Wu
YS Yongfeng Shan
BZ Binbin Zhang
HW Haonan Wang
HL Hui Liu
HY Hao Yu
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With regard to RNase R treatment, the total RNA was cultivated with or without RNase R (Epicentre Technologies) for half an hour at RT. As for ActD treatment, cells were supplemented to the ActD (Sigma‐Aldrich) or control DMSO (Sigma‐Aldrich) for indicated times (0, 4, 8, 12 h). Circ_NEK6 and the linear NEK6 in indicated cells were analyzed via RT-qPCR.

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