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Carbenoxolone Disodium

KS Katia Suarez-Berumen
HC Henry Collins-Hooper
AG Anastasia Gromova
RM Robyn Meech
AS Alessandra Sacco
PD Phil R. Dash
RM Robert Mitchell
VS Valery I. Shestopalov
TW Thomas E. Woolley
SV Sakthivel Vaiyapuri
KP Ketan Patel
HM Helen P. Makarenkova
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Primary myoblasts were treated with carbenoxolone (CBX, Sigma-Aldrich # C4790) or vehicle, for 24 h prior and then induced to differentiate in low serum medium also containing CBX or vehicle (control). CBX blocks pannexin and connexin channels at 100 μM, but has high selectivity for pannexin channels at 10–50 μM (Bruzzone et al., 2005). Varying CBX concentrations from 10 to 100 μM were tested and 25 μM was found to be optimal for pannexin inhibition. Myoblast cultures treated with 25 μM CBX in DM were fixed at different time points 6, 12, 24, and 72 h and processed for immunostaining.

Copyright and License information:  ©2021 Suarez-Berumen, Collins-Hooper, Gromova, Meech, Sacco, Dash, Mitchell, Shestopalov, Woolley, Vaiyapuri, Patel and Makarenkova.
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