Wire Hang Test

The wire hang test was also performed (Luk et al., 2012a). To summarize this, the mice were hanged on a 55-cm-long 2-mm-thick wire at a height of 90 cm. Four initial training sessions composed of 5-min hang and 5-min break were conducted. After an hour, the test trials were conducted, and the latency of mice to fall off the wire was recorded. Each mouse should stay on the wire for at least 10 s, and animals not falling off after 300 s were given a maximum score of 300 s (≤10 s x ≤300 s). The average values from the three trials were then used for analysis.