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The modeled structure of the SMP domain of PDZD-8 (Supplementary Fig. 7l) was obtained by submitting the primary sequence (residues 38–340) of PDZD-8 (coding cDNA, C53B4.4b.1) to the I-TASSER server129, using the SMP domain of E-Syt2 structure (PDB: 4P42)65 as a template. The modeled structure of the SMP domain of PDZD-8 with mutated residues (L98W, I252W) were generated using the “Mutagenesis” function of the PyMOL.
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