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BrdU incorporation assay

YJ Young-Jun Ju
SL Sung-Woo Lee
YK Yoon-Chul Kye
GL Gil-Woo Lee
HK Hee-Ok Kim
CY Cheol-Heui Yun
JC Jae-Ho Cho
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To analyze proliferation ability upon LCMV challenge, indicated mice were i.p. injected with BrdU (1 mg/mice) 2 h before sacrifice. BrdU staining was performed with eBioscience BrdU Staining Kit for Flow Cytometry FITC (eBioscience) according to the manufacturer’s protocol. In brief, splenocytes were stained with surface markers, then fixed and treated with DNase I. Cells were stained with fluorochrome conjugated anti-BrdU antibody and analyzed with flow cytometry. To analyze self-renewal capacity, indicated mice were i.p. injected with BrdU (1 mg/mice) once then treated with BrdU (0.8 mg/ml) in their drinking water for 7 days before sacrifice. BrdU solution was prepared in sterile water and protected from light. BrdU staining was performed as previously described.

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