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Flow cytometry to detect apoptosis

LZ Lin Zhou
ZH Zhi Huang
SY Shanyi Yang
JW Jiarui Wei
YX Yan Xu
LH Lin Hu
XG Xinrong Guo
LY Limin Yuan
ZY Zexuan Yuan
XY Xiaoping Yang
XT Xiaojun Tao
QZ Qiufang Zhang
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To further investigate the effects of different ICA nanoparticles on LPS-induced cell apoptosis, we used the Annexin V-FITC/PI double staining method to analyze the apoptotic rate. After H9c2 treatment as described above, cells were harvested and resuspended in 195 μL of binding buffer containing 10 µL of Annexin V-FITC and 5 µL of PI and then incubated for 15 min at room temperature in the dark. After centrifugation at 1500 rpm for 10 min at 4 °C, the staining buffer was aspirated, and the cells were washed twice with PBS and resuspended in 100 µL of PBS. Finally, the samples were measured by flow cytometer.

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