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2.4. Skimmed milk flocculation concentration
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Most of the Moore swab samples were processed by the skimmed milk method (Melgaco et al., 2016). Briefly, a 5% (w/v) skimmed milk solution was prepared by dissolving 5 g of skimmed milk powder (BD, #232100, Sparks, MD) in 100 mL of distilled water. Before the flocculation step, the wastewater sample pH was adjusted to 3.5 using 6 N HCL and then skimmed milk was added at a final concentration of 1%, followed by addition of 105 EGC of BRSV and shaking for 2 h.
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