The transverse sections of paraffin embedded nerve tissue were used to perform myelin staining with a Luxol fast blue stain kit (ab150675, Abcam, USA) [27]. Briefly, nerve sections were deparaffinized and rehydrated in distilled water. The slides were incubated at room temperature in Luxol fast blue solution for 14–16 h, rinsed with distilled water, and dipped in lithium carbonate solution for 20 s. Until visible myelin sheath structure was obtained (2.5 min), the additional background color was removed by repeated dipping in 70% alcohol solution. After rinsing in water and dehydration in 100% alcohol, the slides were examined under a 40 × objective lens with the microscope, and images were obtained.
Copyright and License information: The Author(s) ©2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this
article to respond.