Myelin sheath staining by Luxol fast blue solution

AY Anjali Yadav
TH Tzu-Chieh Huang
SC Szu-Han Chen
TR Thamil Selvee Ramasamy
YH Yuan-Yu Hsueh
SL Shau-Ping Lin
FL Fu-I Lu
YL Ya-Hsin Liu
CW Chia-Ching Wu
request Request a Protocol
ask Ask a question
Favorite

The transverse sections of paraffin embedded nerve tissue were used to perform myelin staining with a Luxol fast blue stain kit (ab150675, Abcam, USA) [27]. Briefly, nerve sections were deparaffinized and rehydrated in distilled water. The slides were incubated at room temperature in Luxol fast blue solution for 14–16 h, rinsed with distilled water, and dipped in lithium carbonate solution for 20 s. Until visible myelin sheath structure was obtained (2.5 min), the additional background color was removed by repeated dipping in 70% alcohol solution. After rinsing in water and dehydration in 100% alcohol, the slides were examined under a 40 × objective lens with the microscope, and images were obtained.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A