Pre-miR miRNA mimic and anti-miR miRNA inhibitor transfection

CR C. G. H. Rönnau
SF S. Fussek
FS F. P. Smit
TA T. W. Aalders
OH O. van Hooij
PP P. M. C. Pinto
MB M. Burchardt
JS J. A. Schalken
GV G. W. Verhaegh
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Cell lines were transiently transfected with 20–50 nM of pre-miR miRNA precursors (‘mimics’) and anti-miR miRNA inhibitors (Ambion; Supplementary Table 2). For transfection, Lipofectamine RNAiMAX transfection reagent (Life Technologies) was used, according to the manufacturer’s instructions.

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