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Rapidly autopsied, frozen human temporal cortex tissues were obtained from the Joseph and Kathleen Bryan Brain Bank (Duke University). Twenty-five subjects were selected with post-mortem intervals (PMI) of <13 h. The project was approved by the Duke Institutional Review Board (IRB). RNA Integrity Number (RIN) evaluation was done using RNA extracts from brain tissue. Brain samples that showed RIN values >7 were selected for conducting the following steps of the method introduced in this paper.
Copyright and License information: ©2016 Tagliafierro, Bonawitz, Glenn and Chiba-Falek.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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