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MN Mina Ekrami Noghabi
MS Mohammad Jafar Saffar
SR Shaghayegh Rezai
HS Hana Saffar
HS Hiva Saffar
FH Fatemeh Hosseinzadeh
AG Aliasghar Nadi Ghara
MR Mohammad Sadegh Rezai
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The objective of this study was to determine antibody seroprotection rates (anti-HBs, anti-Hib PRP, anti-diphtheria toxoid, antitetanus toxoid, and anti-Bordetella pertussis) and calculating geometric mean concentrations (GMC) before vaccination and 4 weeks after the third dose.

Antibody titers were measured by ELISA quantitative kits as follows: Diphtheria (Eurofins Co., with seroprotection defined as antibody concentration <0.01 IU/mL considered as unimmune, 0.01–0.1 IU/mL requiring booster dose and >0.1 IU/mL immune); Tetanus (Eurofins Co., <0.1 IU/mL unimmune, 0.1–1 IU/mL requiring control after 1 to 2 years, 1–5 IU/mL requiring control after 2-4 years and >5 IU/mL requiring control after 4–8 years); Pertussis (Eurofins co., <16 IU/mL unimmune, 16–24 IU/mL borderline and > 24 IU/mL immune); HepB (Diapro Co, <10 IU/mL unimmune, and >10 IU/mL immune); Hib (Demeditec Co, <0.15 IU/mL unimmune, and >0.15 IU/mL immune).

Serum isolated from blood samples were stored at a temperature of−20 °c and analyzed after collection.

Copyright and License information:  ©2021 Ekrami Noghabi, Saffar, Rezai, Saffar, Saffar, Hosseinzadeh, Nadi Ghara and Rezai.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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