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IHC staining was performed on formalin-fixed and paraffin-embedded tissues or paraformaldehyde-fixed cells. After being treated with the blocking solution (0.1% Triton-X) for penetration, fixed tissues or cells were incubated with anti-GLI1 antibodies overnight at 4°C, followed by an incubation with the secondary antibody. Images were collected using a digital camera (Nikon, Japan).
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