In vivo antitumor efficacy

Twenty-five (five per group) STOCK-Foxn1nu/Nju mice were subcutaneously injected with 5 × 10⁶ SW480 cells on day 0 to establish a xenograft tumor model. On days 7 and 14, the mice were intravenously treated with 3.0 × 10⁶ PD-L1-CAR-T cells, CCSC-DC vaccine-sensitized T cells, both PD-L1-CAR-T cells and CCSC-DC vaccine-sensitized T cells, normal T cells, or PBS. Tumor growth was monitored and measured using Vernier calipers every third day. One month later, surgery was conducted to remove the tumors. A caliper was used to measure the length and width of the tumor, and the tumor volume was approximated as follows: 1/2 × length × (width)².