Cell proliferation assay (CCK-8)

A cell-free medium was used as the blank control group. In the experimental group, resistin (10-40 ng/mL) and rapamycin 30 nM were added and incubated for 24, 48, and 72 h at 37 °C. After 24 h of incubation, the medium was aspirated, and 10 mL of CCK-8 solution was added to each well. After incubating the culture for another 3 h, a microplate reader was used to detect the absorbance of the samples at 450 nm. The cell proliferation rate was calculated by measuring the optical density of the cell culture at 450 nm and plotting a growth curve.