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Histological and histomorphometric analysis

XW Xuesong Wang
ZN Ziyan Nie
JC Jia Chang
ML Michael L. Lu
YK Yunqing Kang
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All harvested samples were processed for histological analysis by decalcifying with a rapid decalcifier solution (RDO Rapid Decalcifier, IL) for 7 days until the samples were softened. The samples were then embedded with paraffin and sectioned laterally and transversely. Hematoxylin and eosin (H&E) staining was carried out to evaluate newly formed bone density and height. For newly formed bone density analysis, each implant was analyzed from proximal, middle, and distal three transversal segments, and 0.5 mm height for each segment (bottom, middle, top). Three different regions of each segmental section were calculated, and three samples of each type of implant was considered. For all the other semi-quantitative evaluations, only central lateral segmental sections were considered for each implant.

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