4.3.2. Synthesis of HAMA, CSMA, and Chondroitin Methacrylate Functionalized with Methacrylic Anhydride

A method for synthesis of HAMA functionalized with methacrylic anhydride in a water/DMF solution adapted by Abbadessa et al.⁶ was used to synthesize HAMA, CSMA, and chondroitin MA of various DM. For HAMA, methacrylic anhydride was added in molar feed ratios of 2.2:1 (anhydride/HA disaccharide). For the synthesis of CSMA and ChMA, the same procedure and concentrations as for the synthesis of HAMA were performed, with a molar feed ratio of 3:1 (anhydride/CS disaccharide or anhydride/Ch disaccharide) for both reactions. The DMs of the obtained HAMA, CSMA, and ChMA were determined using the HPLC method described below.