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Flow cytometry assay in the presence of EDTA.

EW Emily E. Whittle
HM Helen E. McNeil
ET Eleftheria Trampari
MW Mark Webber
TO Tim W. Overton
JB Jessica M. A. Blair
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Growing culture samples were taken at 1, 3, and 5 h as described above. Samples were made with various concentrations of EDTA (0 μM, 1 μM, 10 μM, 100 μM, 200 μM, and 500 μM) in 500 μl HBS. These concentrations of EDTA increased the final volume of the sample because the stock concentration was limited by solubility. Dyes were then added, but the volume added was adjusted to maintain the final concentrations stated above. Once the dyes were added, 100 μl of cell suspension was added and cells were incubated for 10 min at room temperature. Samples were then analyzed by flow cytometry.

Copyright and License information:  ©2021 Whittle et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.This content is distributed under the terms of the Creative Commons Attribution 4.0 International license.

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