2.7. Acetaminophen (AAP) Induced Hepatotoxicity in Hepg2 Cells

To evaluate the hepatoprotective effects of the sage essential oils on HepG-2 cell lines, the non-toxic concentration of the sage essential oil, 100 μg/mL, was selected to conduct the hepatoprotective activity assessment using the MTT assay as described earlier (Section 2.6). The HepG-2 cell lines were treated with AAP (4 mM) for 24 h, and the HepG-2 cells in the medium were considered as a negative control. Further, the hepatoprotective assays were performed using standard methods as described in each assay in the following sections as detailed below. The HepG-2 cells were pretreated with the essential oils for 12 h and incubated with AAP (4 mM) for 24 h, with and without the essential oils; the HepG-2 cells in medium only were considered as a negative control.