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Melanocytes were dissolved in 200 μL of 1 N NaOH (30 min, 100 °C) and the concentration of melanin was quantified by measuring the absorbance at 405 nm, as described previously [44]. The calculated melanin content was adjusted based on the cell number.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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