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After osteosarcoma cells were seeded into 6-well plates and incubated overnight, they were treated with Cis (20 μmol/L), Dox (0.2 μg/mL), or Mtx (50 μmol/L) for 24 h. Apoptotic cells were determined using an Annexin V-PE Apoptosis Detection kit (BD, Shanghai, China) by flow cytometry according to the manufacturer’s instructions.
Copyright and License information: ©2021 Tang, Wei, Li, Wang, Wu, Dong, Liu, Wei, Shi, Li, Guo and Xiao.
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