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Nanoparticle dispersion and washing

CS Cassandra M. Stawicki
TR Torri E. Rinker
MB Markus Burns
ST Sonal S. Tonapi
RG Rachel P. Galimidi
DA Deepthi Anumala
JR Julia K. Robinson
JK Joshua S. Klein
PM Parag Mallick
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To ensure that nanoparticles are well-distributed in an aqueous solution, tubes of particles were dispersed by pipetting up and down while partially immersed in a sonication bath (Branson Bransonic Ultrasonic Cleaner 8510R-DTH). Nanoparticle washes were performed as follows: (1) nanoparticles were centrifuged at 31,000 × g for 30 min (pre-PEGylation) or 60 min (post-PEGylation) in a 1.5-mL tube; (2) supernatant was removed, taking care not to disturb the pellet; (3) appropriate volume of buffer was added; and (4) the pellet was redispersed by pipetting up and down while sonicating until no large aggregates of nanoparticles were visible.

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