Phylogenetic Analysis

The amino acid sequences of DIR proteins from mungbean and other species reported by Corbin et al. (Corbin et al., 2018), including DIR members from Arabidopsis thaliana, Forsythia x intermedia, Gossypium barbadense, Glycyrrhiza echinata, Gossypium hirsutum, Hordeum vulgare, Oryza sativa, Podophyllum peltatum, Picea sitchensis, Sorghum bicolor, Schizandra chinensis, Saccharum hybrid cultivar, Sesamum indicum, Triticum aestivum, Tamarix androssowii, Tsuga heterophylla, Thuja plicata, Linum usitatissimum, Zea mays, Glycine max, Vigna unguiculata, Pisum sativum and Phaseolus vulgaris were aligned using ClustalW2 (Oliver et al., 2005). The phylogenetic tree was constructed using the alignment results of DIR proteins from these species in MEGA 7.0 using the neighbor-joining method with default parameters (Kumar et al., 2016). The classification of mungbean VrDIR genes was carried out as described by Corbin et al. (Corbin et al., 2018).