Cell Death ELISA

Cells were plated at 25,000 cells/well in 24-well plates in complete RPMI 1640 with no selection agent. Cells were treated as indicated 24 hours after plating. Mono- and oligonucleosomes indicative of apoptosis released into the media were detected using the Cell Death Detection ELISA kit (Cat#: 11544675001) purchased from Roche according to the manufacturer’s instructions. Apoptotic cell death was measured at 405 nm absorbance. A cell growth assay on cells in the wells was also performed and apoptosis absorbance values were normalized to cell count. All conditions were performed in triplicate. Experiment was performed twice.