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Statistical analysis and curve fitting were done with prisma 6.0 (GraphPad Software, Inc., La Jolla, CA, USA). All data are presented as means with error bars (means ± SE). In figures, n indicates the number of independent experiment performed. Differences between means were analyzed using Student's t‐test for paired or unpaired data, wherever appropriate with a P value of < 0.05 taken as statistically significant. Inhibition and saturation data were analyzed by nonlinear regression. Hanes–Wolf plots were used to visually display the results.
Copyright and License information: ©2016 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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