Isolation of bone marrow derived macrophages (BMMs)

BMMs were prepared from the femurs and tibia of 3-month-old C57BL/6j mice. Briefly, the mice were sacrificed, femurs and tibia were dissected. BMMs were flushed with syringe with 24 G needle containing differentiation media, which include macrophage-colony stimulating factor (M-CSF) as previously described (Dupont et al., 2011). The cells were fed with fresh M-CSF media every 2 to 3 days. After this, the cells were utilized for the siRNA knockdown experiments.