Scanning electron microscopy of the Drosophila eye

Several single vial crosses were made at 29 °C and adult male flies collected upon eclosion and aged for three days before being frozen at −80 °C. Whole flies were mounted on scanning electron microscope stubs, desiccated overnight and photographed with a FEI Mineral Liberation Analyzer 650F scanning electron microscope. For each cross at least 10 eye images were analysed using the National Institutes of Health (NIH) ImageJ software (Schneider, Rasband & Eliceiri, 2012) and biometric analysis performed using GraphPad Prism version 5.04. The percent area of eye disruption was calculated as previously described (M’Angale & Staveley, 2012).