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Probe-level data were subject to quality control using ArrayQualityMetric Bioconductor package (23), followed by RMA background adjustment with quantile normalization and log2 transformation. Probe sets were summarized by median polish. For significance assessment, expression differences between baseline and the day of interest were calculated for each subject, and difference of mean difference across all subjects from zero was assessed by one-sample Student t test.
Copyright and License information: ©2016
Freely available online through the PNAS open access option.
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