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Beam Walking Test

LC Lin Chen
SR Shu-Yu Ren
RL Rui-Xue Li
KL Kun Liu
JC Jing-Fei Chen
YY Yu-Jian Yang
YD Yong-Bin Deng
HW Han-Zhi Wang
LX Lan Xiao
FM Feng Mei
FW Fei Wang
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A modified beam walking test was used to assess the motor coordination of mice. In the test, a beam (0.4 cm wide) was placed 50 cm above the floor in a dark room, with one end illuminated by a lamp and the other end in a non-transparent box (20 × 20 × 20 cm3). Each mouse was placed at the end with light and trained three times per day for 3 days before testing. In order to attract the mouse to walk across the beam in a sequence of 30-, 50- and 70-cm distances, padding material from its cage was placed in the box during the training days. During the test, videos were collected from both sides of the beam to record the performance. The frequency of foot-slips when the mouse walked a distance of 80 cm was calculated as the index of beam walking performance [25].

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