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Generation of cell lines with acquired resistance to EGFR-TKIs
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All resistant cells were established by chronic, repeated exposure to gefitinib or osimertinib, as reported in previous studies28,30. For all experiments, resistant cells were cultured in a drug-free medium for at least 1 week to eliminate gefitinib or osimertinib. Gefitinib- and osimertinib-resistant cells are referred to as PC-9/GR and H1975/OR cells, respectively.
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