Delayed Type Hypersensitivity

TG T. T. Guimarães
SG S. M. R. Gomes
RA R. A. A. C. Albuquerque
AL A. K. C. Lima
GB G. F. Braga
JS J. B. Souza
MA M. Assis
AB A. C. S. Brito
RS R. F. Santos
TS T. Da Silva
LS L. M. Siqueira
BV B. D. Ventura
LR L. S. Rodrigues
RT R. Terra
SS S. A. G. Da Silva
PD P. M. L. Dutra
request Request a Protocol
ask Ask a question
Favorite

To evaluate delayed type hypersensitivity (DTH), 20 μg of the total antigen (in 20 μl) was inoculated into the plantar cushion of non-infected paw. After 48 h from the inoculation, the extent of swelling was measured using a caliper rule (Mitutoyo). The DTH was expressed as the difference between the paw thickness before and after the antigen inoculation.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A