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NO levels from media collected from RAW264.7 macrophages were quantified by Griess Reagent System according to manufacturer’s instructions (Promega, Madison, WI, United States). Briefly, 50 μl of supernatant or standards were added to a 96-well plate, followed by 50 μl sulfanilamide solution and 50 μl N-1-napthylethylenediamine dihydrochloride (NED) solution, with a 10 min incubation period after the addition of each. Absorbance at 550 nm was measured using a Clariostar Monochromator Microplate Reader (BMG Labtech, Germany).
Copyright and License information: ©2021 Chan, Wong, Lee, Leung, Shum, Cho, Chen and Tai.
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