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Measurement of mouse lipid contents
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Lipids were extracted from liver samples using Folch solution composed of a 2:1 (vol/vol) mixture of chloroform/methanol as described previously56. Lipids were solubilized in 10% Triton X-100 before evaporation. Non-esterified fatty acid (Fujifilm/Wako, Lexington, MA, USA), cholesterol (Stanbio, Boerne, TX, USA), and triglyceride content (Stanbio, Boerne, TX, USA) were measured in hepatic extracts and plasma samples using colorimetric assays.
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