RAW 264.7 macrophage cell culture

ES Emily C. Suter
ES Eva M. Schmid
AH Andrew R. Harris
EV Erik Voets
BF Brian Francica
DF Daniel A. Fletcher
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RAW 264.7 murine male macrophage-like cell line was obtained from the UC Berkeley Cell Culture Facility. Cells were cultured in RPMI 1640 media (Corning) supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS, Thermo Fisher Scientific) and 1% Pen-Strep (Thermo Fisher Scientific) in non-tissue culture-treated 10 cm dishes (VWR) at 37°C, 5% CO2.

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