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The minimum inhibitory concentration (MIC) of the proteins was determined in triplicate by the conventional broth microdilution technique in TSB (CLSI, 2015). In this assay twofold dilutions of each protein were added to a microtiter plate containing 105 CFU/well living staphylococcal cells. The MIC was defined as the lowest protein concentration that inhibited visible bacterial growth after 24 h of incubation at 37°C. The final MIC values for each protein and strain correspond to the mode of three independent biological repeats.
Copyright and License information: ©2021 Gutiérrez, Rodríguez-Rubio, Ruas-Madiedo, Fernández, Campelo, Briers, Nielsen, Pedersen, Lavigne, García and Rodríguez.
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