Superoxide Radical (•O2-) Scavenging Assay

The inhibition of superoxide anion radical kits has biological significance by simulating the reaction system of xanthine and xanthine oxidase to generate superoxide anion radicals. In this experiment, the reagents were prepared according to the kit instructions, different concentrations of TSAT solution (0.01–1 mg/mL) were added, and a water bath at 37 °C for 40 min. VC was used as a positive control. The scavenging rate (%) was calculated as follows:

where A₀ represents the absorbance of the negative control with deionized water instead of the sample; A₁ represents the test system containing the sample; and A₂ is the absorbance of the blank system. The IC₅₀ value was then calculated. All operations were performed in parallel three times.