Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Confirmation of carbapenemase production

SP Susil Pyakurel
MA Mehraj Ansari
SK Smriti Kattel
GR Ganesh Rai
PS Prasha Shrestha
KR Kul Raj Rai
AS Anil Kumar Sah
SR Shiba Kumar Rai
ask Ask a question
Favorite

Carbapenemase production was confirmed phenotypically by the Modified Hodge Test (MHT). For this, an overnight suspension of E. coli (ATCC 25922) adjusted to a turbidity of the 0.5 McFarland standard was inoculated evenly on the surface of the MHA plate containing 70 μg per ml of ZnSO4. After drying at room temperature, meropenem was placed at the center of the plate, and then the screening positive isolates were stroked from the edge of the disk to the periphery of the plate and incubated overnight at 37 °C aerobically. The presence of a clover leaf-shaped inhibition zone was considered carbapenemase production. The confirmed isolates were preserved in 25% glycerol and stored at − 20 °C until further confirmation by molecular test.

Copyright and License information: The Author(s) ©2021
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A