2.2. Myocardial Infarction Model

Male Wistar rats (n = 40), weighing 250–280 g of body weight (b.w.) and fed ad-libitum, were injected subcutaneously (s.c.) with (−)-isoproterenol hydrochloride (ISO) at a dose of 70 mg/kg b.w, between 09:00 and 10:00 h (n = 25), whereas control rats (n = 15) received an equivalent volume of saline solution. Animals were euthanized after 3 and 6 h (pre-infarction period), 24 h (infarction), and 48 h and 96 h (post-infarction period) of ISO administration [6]. Our research was approved by the Animal Experiments Institutional Ethics Committee (Protocol code = CICUAL RHM134-18), according to the Federal Regulations for Animal Experimentation (Ministry of Agriculture, SAGARPA, Mexico).