4.6. Cell Viability Assay

Cell viability was assessed using CellTiter-Glo (Promega, Southhampton, UK) luminescent assay. Briefly, cells were seeded at 2 × 10⁵/mL in a 12-well plate, appropriate drug treatments were added and the plates were incubated in a humidified incubator at 37 °C supplemented with 5% CO₂ for up to 72 h. Following a 72 h incubation period, cell culture medium from each well was added to an equal volume of CellTiter-Glo reagent in a white 96-well plate. The plate was mixed on an orbital shaker for 2 min to induce cell lysis followed by a 20 min incubation at room temperature to stabilise the luminescent signal. Luminescence was measured using a Synergy HTX Multi-mode Microplate reader (Biotek, Winooski, VT, USA).