3.2. Isolation of Cypermethrin 95% Degrading Bacteria

The cultivated soil sample collected from El-Menofia Governorate (GPS: 30°30′22.45″ N; 31°0′15.3″ E) was used for bacterial isolation. The collected soil sample has a history of chemicals spray including herbicides, insecticides, and nematicides. The isolation procedure was achieved using MS agar media supplemented with 500 ppm of cypermethrin as a sole carbon and energy source. Under aseptic condition, one milliliter of diluted sample (10³) was spread over the surface of Petri dishes of the prepared MS agar media and incubated at 35 °C for seven days.

The different bacterial colonies that appeared on the Petri plates were picked up and reinoculated again onto a new MS agar plate to obtained purified isolates which were preserved onto MS agar slants and preserved at −4 °C for further work [75].