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2.6. Caspase-3/7 Activity

IM Ilandarage Menu Neelaka Molagoda
MK Mirissa Hewage Dumindu Kavinda
YC Yung Hyun Choi
HL Hyesook Lee
CK Chang-Hee Kang
ML Mi-Hwa Lee
CL Chang-Min Lee
GK Gi-Young Kim
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HaCaT keratinocytes were treated with fisetin (0–10 μM) for 2 h prior to exposure to 100 μg/mL PM2.5 for 24 h. The cells were subsequently harvested and stained with a Muse Caspase-3/7 Assay Kit (Luminex), following which the cells were incubated with 7-aminoactinomycin D (7-AAD) for detecting the apoptotic cells. The population of caspase-3/7+ apoptotic cells was measured using a Muse Cell Analyzer.

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