2.3. Cell Migration Assay Using Scratch Method

SK Seon-Young Kim
SP Song-Yi Park
HJ Hwan-Seok Jang
YP Yong-Doo Park
SK Sun-Ho Kee
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Cell migration was induced by scratching the cells at about 90% confluence with a pipette tip and replacing them with fresh 10% FBS containing medium. After scratching, cells were photographed at indicated time points using a Dino-Eye Digital Eyepiece Camera (Dino-Lite, Taiwan), and the change in scratch area was measured using Image J software (NIH, Bethesda, MD, USA).

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