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Erythrocyte catalase (e-CAT) activity

AN A Noce
MV M F Vidiri
GM G Marrone
EM E Moriconi
AB A Bocedi
AC A Capria
VR V Rovella
GR G Ricci
AL A De Lorenzo
ND N Di Daniele
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e-CAT activity was determined with a spectrophotometric assay at 240 nm (25 °C), using a Kontron Uvikon 941 Plus spectrophotometer (Kontron Instruments). One volume of 5 μl of hemolyzed blood was diluted in 1 ml of potassium phosphate buffer 0.05 M pH 7.0 with ethylenediaminetetraacetic acid 0.1 mM, and finally 10 ul of H2O2 1 M according to the standard procedure of Beers and Sizer.47 Results were expressed as enzyme units (U) per gram of Hb (U/gHb): one unit represents the amount of enzyme that catalyzes the decomposition of 1 micromole of H2O2 in 1 min at 25 °C.

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